Notebook: Yeast Courses, 1983

Display Title

Notebook: Yeast Courses, 1983

Source

box: 11 folder: 01-02 (text)

Extent

2 Folders

Description

This a notebook within a series involving Grieder's course work at UC Berkley. The notebook begins with a guest lecture by J. Thorner. The course professor gave the class a question on containing cells in a plasmid. The notebook also contains a letter from Bruce Stillman to Grieder. Grieder apologized for slow response to her letters regarding grant proposals. Stillman reponds and tells Grieder that he will telephone Grieder in a couple of days. The notebook also contains a course diagram of spindle pole bodies and questions from lecture, which she received 98%.

It also included an unscheduled DNA synthesis and a recombination diagram. She disagreed with the diagram because she believed that each of the structures could be inherited and each could initiate replication once in the next double structure shown. She also created her own diagram and model which described what the model could predict.

In another course, entitled: Molecular Genetics of Yeast- taught by course instructor Alvin Clark in 1983. The course covered genetic and specific aspects of moleculara genetics of Saccharomyces cerevisiae. The course also included review articles for the students as well as experimental articles. It also included a diagram from textbook on nuclear organization and cell-division. The textbook also had diagrams of the cell cycle of yeast and its conjugation. The notebook also included diagrams and explanations of its cell wall and septum formation during the yeast cell cycle along with a tentative functional sequence map of the yeast cell cycle on the next page. On another page there is a diagram of a resting cell of bakers yeast. Grieder noted on the side certain organelles of yeast cell like its nucleus, vacuole, and Golgi apparatus.

Grieder diagrammed out the effects of recombination the segregation of linked genes. The diagram included the cell cycle, specifcally the 1st Meiotic Prophase and 2nd Meiotic Prophase with genotype and phenotypes.

There are also copious notes on yeast cell analysis, with Grieder noting that the cell wall of yeast cells are thicker and more porous. The cell wall of the yeast organism is composed of glycoprotein and the protien chitin. The chitin and glycoprotien seemed to protect cell from osmotic lysis. It contained hydrolytic enzymes
and seemed to be used for growth and mating. She needed to understand cell structure in order to do spheroplast studies, where Grieder removes the cell wall. The studies also contained documents from class and articles on the general overview of Transcriptase in Yeast and specific examples of regulatory sequences within 5 prime flanking regions of yeast structural genes.

In another document, take home problem from Dr. Alvin Clark's course on Yeast, asking how DNA sequences mediate and control the actions of regulatory sequences.

There is another series of documents on mating type switching. The series shows different models and methods for switching. Grieder showcases several examples such as switching phenotypes among different strains. The models include: flip-flopping, cassete, and double strand. It also included an electron micrograph of yeast as well as a drawing of budding and separation of the yeast model organism.

Grieder also asked rather interesting questions regarding yeast, specifically on chromosomal influence on telomeres. One of the questions was regarding how chromosomes moniter length inside the nucleus.

The notebook concludes with different mapping techniques for yeast cultures as well as diagrams of its life cycle.

Scope and Contents

This a notebook within a series involving Grieder's course work at UC Berkley. The notebook begins with a guest lecture by J. Thorner. The course professor gave the class a question on containing cells in a plasmid. The notebook also contains a letter from Bruce Stillman to Grieder. Grieder apologized for slow response to her letters regarding grant proposals. Stillman reponds and tells Grieder that he will telephone Grieder in a couple of days. The notebook also contains a course diagram of spindle pole bodies and questions from lecture, which she received 98%.

It also included an unscheduled DNA synthesis and a recombination diagram. She disagreed with the diagram because she believed that each of the structures could be inherited and each could initiate replication once in the next double structure shown. She also created her own diagram and model which described what the model could predict.

In another course, entitled: Molecular Genetics of Yeast- taught by course instructor Alvin Clark in 1983. The course covered genetic and specific aspects of moleculara genetics of Saccharomyces cerevisiae. The course also included review articles for the students as well as experimental articles. It also included a diagram from textbook on nuclear organization and cell-division. The textbook also had diagrams of the cell cycle of yeast and its conjugation. The notebook also included diagrams and explanations of its cell wall and septum formation during the yeast cell cycle along with a tentative functional sequence map of the yeast cell cycle on the next page. On another page there is a diagram of a resting cell of bakers yeast. Grieder noted on the side certain organelles of yeast cell like its nucleus, vacuole, and Golgi apparatus.

Grieder diagrammed out the effects of recombination the segregation of linked genes. The diagram included the cell cycle, specifcally the 1st Meiotic Prophase and 2nd Meiotic Prophase with genotype and phenotypes.

There are also copious notes on yeast cell analysis, with Grieder noting that the cell wall of yeast cells are thicker and more porous. The cell wall of the yeast organism is composed of glycoprotein and the protien chitin. The chitin and glycoprotien seemed to protect cell from osmotic lysis. It contained hydrolytic enzymes
and seemed to be used for growth and mating. She needed to understand cell structure in order to do spheroplast studies, where Grieder removes the cell wall. The studies also contained documents from class and articles on the general overview of Transcriptase in Yeast and specific examples of regulatory sequences within 5 prime flanking regions of yeast structural genes.

In another document, take home problem from Dr. Alvin Clark's course on Yeast, asking how DNA sequences mediate and control the actions of regulatory sequences.

There is another series of documents on mating type switching. The series shows different models and methods for switching. Grieder showcases several examples such as switching phenotypes among different strains. The models include: flip-flopping, cassete, and double strand. It also included an electron micrograph of yeast as well as a drawing of budding and separation of the yeast model organism.

Grieder also asked rather interesting questions regarding yeast, specifically on chromosomal influence on telomeres. One of the questions was regarding how chromosomes moniter length inside the nucleus.

The notebook concludes with different mapping techniques for yeast cultures as well as diagrams of its life cycle.

Preferred Citation

Notebook: Yeast Courses, 1983. Carol Greider Collection, Cold Spring Harbor Laboratory Archives Digital Repository. 88-1525529. Update 2025-03-18.

Credit Line

Courtesy of Cold Spring Harbor Laboratory Archives.

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Level

file

Collection

Carol Greider Collection

Resource ID

CWG

Reference ID

5f0592fd32d0631e257ee76b54eeaeaa

Finding Aid Link

Notebook: Yeast Courses