Course Notebook: Methods DNA II, 1984 - 1989

Display Title

Course Notebook: Methods DNA II, 1984 - 1989

Source

box: 09 folder: 01 (text)

Description

This is a notebook within a series on the methods that Grieder used in her experiments. The notebook begins with a method to isolate DNA fragments by spinning nucleate and using agarose gel electrophoresis. As well as different methods of extraction that were used in previous experiments like glass beads. Also included a southwestern blotting technique, which created different transfer buffers and binding buffers.

The notebook also contained an interesting letter from Jillian, a colleague, to Greider regarding steps to take in DNA preps. As well as detailed instructions for mini RNA preps. Also included a method of generating a high volume of oligonucleotide probes and a protocal book for BRL's (Bethesda Research Laboratories) RNA Sequencing System. The cover of the book has referenced certain articles that have used the system. It also included an introduction, a table of contents, and methods for using different sequencing systems for the technology.

The technologies that Greider used ranged from primer extensions to concentrating mRNA molecule. Alongside the methods were articles that have been successful with these procedures. The notebook contained a published article on ribonuclease protection and a letter from a colleague of Greider's, Dave Brow. Brow was giving Greider certain protocols for sequencing RNA using primer extensions. The process seemed to have used reverse transcriptase.

The notebook concluded with articles that used methods and materials that Greider had used in her research, ranging from RNA sequencing to labelling.

Scope and Contents

This is a notebook within a series on the methods that Grieder used in her experiments. The notebook begins with a method to isolate DNA fragments by spinning nucleate and using agarose gel electrophoresis. As well as different methods of extraction that were used in previous experiments like glass beads. Also included a southwestern blotting technique, which created different transfer buffers and binding buffers.

The notebook also contained an interesting letter from Jillian, a colleague, to Greider regarding steps to take in DNA preps. As well as detailed instructions for mini RNA preps. Also included a method of generating a high volume of oligonucleotide probes and a protocal book for BRL's (Bethesda Research Laboratories) RNA Sequencing System. The cover of the book has referenced certain articles that have used the system. It also included an introduction, a table of contents, and methods for using different sequencing systems for the technology.

The technologies that Greider used ranged from primer extensions to concentrating mRNA molecule. Alongside the methods were articles that have been successful with these procedures. The notebook contained a published article on ribonuclease protection and a letter from a colleague of Greider's, Dave Brow. Brow was giving Greider certain protocols for sequencing RNA using primer extensions. The process seemed to have used reverse transcriptase.

The notebook concluded with articles that used methods and materials that Greider had used in her research, ranging from RNA sequencing to labelling.

Preferred Citation

Course Notebook: Methods DNA II, 1984 - 1989. Carol Greider Collection, Cold Spring Harbor Laboratory Archives Digital Repository. 88-1525522. Update 2025-03-18.

Credit Line

Courtesy of Cold Spring Harbor Laboratory Archives.

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Level

file

Collection

Carol Greider Collection

Resource ID

CWG

Reference ID

8f8586dba319c333ecdc2cd6c284e54d

Finding Aid Link

Course Notebook: Methods DNA II