Laboratory Notebook: Protein Gels, 1987 - 1989

Display Title

Laboratory Notebook: Protein Gels, 1987 - 1989

Source

box: 08 folder: 04 (text)

Description

This is a notebook within a series on Grieder experiments using gel electrophoresis, as well as sperate experiments in other labs outside the Blackburn laboratory. The notebook begins with a discussion of certain gel sequences that she found interesting in the bands they produce, specifically those involving Thymadine, Guanine, and Cytosine. Grieder was trying to figure out what the band is and the needed sequence to find out as the sequences get very little activity and the oligonucleotides did not work well.

In N.S. lab Meeting, Grieider told the lab that she was attempting to determine the specificity of Tetrahymena telomerase in primer binding. This was needed to do series of experiments and utilized quantitative analysis. Grieder also summarized her work in the Ariel lab. In the N.S. lab she decided to use large scale purification, northern analysis, and probing the northern blot with RNA clones.

In the Maria lab Grieder was titrations on a Xenopus, an African clawed frog which at the time was used primarily embryological research. Also tested Rat liver telomerase.

In the Kathy Lab, Grieder utilized purification techniques which she dialyzed Native gel which allowed her to strip most RNA. The Telomerase activity in this organism was more active than that migrated into gel. Detailed the permutations of Telomerase Template as well as the translocating and adding of certain sequences.

In the Neena Lab, Grieder’s goal was to use the mobility of shifting DNA-binding assays in order to determine the specificity of Tetrahymena telomerase in binding of primers.

In Lavina’s lab Grieder summarized her project and decided to take Geron’s human telomerase Polymerase Chain Reaction assay and used it alongside the reconstituted Tetrahymena Telomerase. Grieder outlined the methods of Jennifer’s lab meetings and notes from subsequent lab meetings.

Also Grieder outlined her notes and methods from Lea’s lab meetings. Grieder also spun, inoculated and strained the cells of the organism she was using. Grieder also created a table out of the results alongside telomerase gel shift as well as DNA and RNA crosslinking. In her lab meeting with Lea, Grieder detailed recent results, purification, and her conclusions.

In the Ariel lab meeting Grieder outlined an approach for identifying human telomerase RNA. Grieder also was using a new Polymerase Chain Reaction approach alongside schematics on extracting telomerase from cell through purification. Also presented a poster on the 1992 Gordon Conference on Molecular Genetics. The notebook concluded with an interesting series of documents. Specifically, Grieder made a table of organism, subunit, recognition sequence, and references. Also Grieder included a summary document where she determined the Human Telomerase RNA.

Scope and Contents

This is a notebook within a series on Grieder experiments using gel electrophoresis, as well as sperate experiments in other labs outside the Blackburn laboratory. The notebook begins with a discussion of certain gel sequences that she found interesting in the bands they produce, specifically those involving Thymadine, Guanine, and Cytosine. Grieder was trying to figure out what the band is and the needed sequence to find out as the sequences get very little activity and the oligonucleotides did not work well.

In N.S. lab Meeting, Grieider told the lab that she was attempting to determine the specificity of Tetrahymena telomerase in primer binding. This was needed to do series of experiments and utilized quantitative analysis. Grieder also summarized her work in the Ariel lab. In the N.S. lab she decided to use large scale purification, northern analysis, and probing the northern blot with RNA clones.

In the Maria lab Grieder was titrations on a Xenopus, an African clawed frog which at the time was used primarily embryological research. Also tested Rat liver telomerase.

In the Kathy Lab, Grieder utilized purification techniques which she dialyzed Native gel which allowed her to strip most RNA. The Telomerase activity in this organism was more active than that migrated into gel. Detailed the permutations of Telomerase Template as well as the translocating and adding of certain sequences.

In the Neena Lab, Grieder’s goal was to use the mobility of shifting DNA-binding assays in order to determine the specificity of Tetrahymena telomerase in binding of primers.

In Lavina’s lab Grieder summarized her project and decided to take Geron’s human telomerase Polymerase Chain Reaction assay and used it alongside the reconstituted Tetrahymena Telomerase. Grieder outlined the methods of Jennifer’s lab meetings and notes from subsequent lab meetings.

Also Grieder outlined her notes and methods from Lea’s lab meetings. Grieder also spun, inoculated and strained the cells of the organism she was using. Grieder also created a table out of the results alongside telomerase gel shift as well as DNA and RNA crosslinking. In her lab meeting with Lea, Grieder detailed recent results, purification, and her conclusions.

In the Ariel lab meeting Grieder outlined an approach for identifying human telomerase RNA. Grieder also was using a new Polymerase Chain Reaction approach alongside schematics on extracting telomerase from cell through purification. Also presented a poster on the 1992 Gordon Conference on Molecular Genetics. The notebook concluded with an interesting series of documents. Specifically, Grieder made a table of organism, subunit, recognition sequence, and references. Also Grieder included a summary document where she determined the Human Telomerase RNA.

Preferred Citation

Laboratory Notebook: Protein Gels, 1987 - 1989. Carol Greider Collection, Cold Spring Harbor Laboratory Archives Digital Repository. 88-1525517. Update 2025-03-18.

Credit Line

Courtesy of Cold Spring Harbor Laboratory Archives.

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Level

file

Collection

Carol Greider Collection

Resource ID

CWG

Reference ID

71946cd62e076b1629a1a44420c11e1a

Finding Aid Link

Laboratory Notebook: Protein Gels