Laboratory Notebook: TLE, Early Notebooks/Telomere Cloning, 1984 - 1992

Display Title

Laboratory Notebook: TLE, Early Notebooks/Telomere Cloning, 1984 - 1992

Source

box: 06 folder: 04 (text)

Description

The notebook begins with an interesting note: Greider was taking classes in Biochemistry in 1984. Proceeded right back into experiments. Diluting and spinning cells into pellets and running minipreps. Also is spheroplasting cells, meaning Greider was destroying the outside cell wall with enzyme zymolyase. Greider spun down the nucleate and ran RNAse with nucleate through a gel. Running more gel studies, she found two bands. They seem to be upper and lower plasmid bands. Greider wanted to cut out each one using glass beads. The possibility for there being two bands might be that there are two plasmids there. Greider found that both then are telomeric fragments, or end fragments of differnt sizes. The overall goal of these experiments were to find cells with plasmids that hybridize strongly. She runs agarose gels and modify’s the cloning techniques slightly.

Scope and Contents

The notebook begins with an interesting note: Greider was taking classes in Biochemistry in 1984. Proceeded right back into experiments. Diluting and spinning cells into pellets and running minipreps. Also is spheroplasting cells, meaning Greider was destroying the outside cell wall with enzyme zymolyase. Greider spun down the nucleate and ran RNAse with nucleate through a gel. Running more gel studies, she found two bands. They seem to be upper and lower plasmid bands. Greider wanted to cut out each one using glass beads. The possibility for there being two bands might be that there are two plasmids there. Greider found that both then are telomeric fragments, or end fragments of differnt sizes. The overall goal of these experiments were to find cells with plasmids that hybridize strongly. She runs agarose gels and modify’s the cloning techniques slightly.

Preferred Citation

Laboratory Notebook: TLE, Early Notebooks/Telomere Cloning, 1984 - 1992. Carol Greider Collection, Cold Spring Harbor Laboratory Archives Digital Repository. 88-1525509. Update 2025-03-18.

Credit Line

Courtesy of Cold Spring Harbor Laboratory Archives.

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Category

Lab Notebooks

Level

file

Collection

Carol Greider Collection

Resource ID

CWG

Component Unique Identifier

CWG_01_025

Reference ID

5e83828fcf7bba410c54f080daa3469f

Finding Aid Link

Laboratory Notebook: TLE, Early Notebooks/Telomere Cloning