Laboratory Notebook 5: In Vitro 81-85/FR 42-69/RNA 22-28 , 1986 - 1987

Display Title

Laboratory Notebook 5: In Vitro 81-85/FR 42-69/RNA 22-28
, 1986 - 1987

Source

box: 03 folder: 01-02 (text)

Extent

2 Folders

Description

This section of the notebook discussed telomere elongation activity. The notebook detailed a series of experiments using the technique of fractionalization of RNA sequences. Greider ran the sequences through gels and stained the gels. The gels were characterized the subsequent reactions and purified them. After finishing the experiment, Greider decided that she might use other organisms outside of Tetrahymena.

Greider tried to identify RNA through alternative approaches in order to get another angle on extraction of the RNA strand. One of the most notable elements of the experiment is the fact that she was having problems with this is experiment. Specifically, Grieder had run cloning experiments, and was attempting to determine if the clone corresponded to a full length RNA strand.

In group meeting Greider announced that she didn't have any new data. However, Greider repeated the RNA experiments and indicated that RNA was required for separation but there was no further separation when it purified through the gradient. Greider found that the experiment had very little activity, because she hadn’t settled on a method of cloning RNA. However, the problem was that in the end of experiment Greider had found very little RNA. So Greider tried to find out why certain techniques, like fractionalization from Fr 15, were causing the RNA to die. Many of the documents included varied ingredients to prepare the RNA for different gel studies. Some of the documents discuss varied ways to change the fractioning, and others document ways to remove certain restriction enzymes from the mix.

Greider also extracted cells from mated, vegetated cells, and glycerol gradient. Greider then spun the cells into a pellet. Grieder repeated the glycerol gradient with the pellet and fractionated the glycerol.

The notebook concluded with gel electrophoresis experiments using 4% Acyrl and a Urea medium on a C4A2 strand.

Scope and Contents

This section of the notebook discussed telomere elongation activity. The notebook detailed a series of experiments using the technique of fractionalization of RNA sequences. Greider ran the sequences through gels and stained the gels. The gels were characterized the subsequent reactions and purified them. After finishing the experiment, Greider decided that she might use other organisms outside of Tetrahymena.

Greider tried to identify RNA through alternative approaches in order to get another angle on extraction of the RNA strand. One of the most notable elements of the experiment is the fact that she was having problems with this is experiment. Specifically, Grieder had run cloning experiments, and was attempting to determine if the clone corresponded to a full length RNA strand.

In group meeting Greider announced that she didn't have any new data. However, Greider repeated the RNA experiments and indicated that RNA was required for separation but there was no further separation when it purified through the gradient. Greider found that the experiment had very little activity, because she hadn’t settled on a method of cloning RNA. However, the problem was that in the end of experiment Greider had found very little RNA. So Greider tried to find out why certain techniques, like fractionalization from Fr 15, were causing the RNA to die. Many of the documents included varied ingredients to prepare the RNA for different gel studies. Some of the documents discuss varied ways to change the fractioning, and others document ways to remove certain restriction enzymes from the mix.

Greider also extracted cells from mated, vegetated cells, and glycerol gradient. Greider then spun the cells into a pellet. Grieder repeated the glycerol gradient with the pellet and fractionated the glycerol.

The notebook concluded with gel electrophoresis experiments using 4% Acyrl and a Urea medium on a C4A2 strand.

Preferred Citation

Laboratory Notebook 5: In Vitro 81-85/FR 42-69/RNA 22-28 , 1986 - 1987. Carol Greider Collection, Cold Spring Harbor Laboratory Archives Digital Repository. 88-1525499. Update 2025-03-18.

Credit Line

Courtesy of Cold Spring Harbor Laboratory Archives.

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Category

Lab Notebooks

Level

file

Collection

Carol Greider Collection

Resource ID

CWG

Component Unique Identifier

CWG_01_009

Reference ID

7b8fe6c3983395d1ce7bbd4110180c52

Finding Aid Link

Laboratory Notebook 5: In Vitro 81-85/FR 42-69/RNA 22-28