Laboratory Notebook 4: In Vitro 56-80/FR 16-41/RNA 1-21, 1985 - 1986

Display Title

Laboratory Notebook 4: In Vitro 56-80/FR 20-40/RNA 10-21, 1985 - 1986

Source

box: 02 folder: 03-04 (text)

Extent

2 Folders

Description

This is a notebook within a series regarding experiments that Greider had done at UC Berkeley. The notebook begins with a summary of experiments that Greider had done so far. Greider set up an experiment with mated, spun, and lysed cells. Greider then mixed the cells to create a pellet of nucleate. With the nucleate Greider began testing different extracts for it and set up reactants, specifcally, G4T2 and NTP. Then ran the product through the gel and the bands were fuzzy. In two particular invitro experiments, Greider tested both hot and cold rNTP’s and fit them into tubes and then ran the tubes through a titration.

Greider found that the rNTP was not well incorporated into the base pair pattern of nucleotides in the DNA segement. She began testing different possibilities in order to figure out what the rNTP is doing in the process. In the end Greider ran the segment through a gel. Greider loaded a different gel this time using specific extracts, reactions, nucleotides. After the experiment, Greider tried to determine how much excess DNA she was adding. She concluded the notebook by reviewing the different preparations to extract DNA. Greider showcased the measurements of buffer solutions, reactants, cell mating processes, and suspension.

Scope and Contents

This is a notebook within a series regarding experiments that Greider had done at UC Berkeley. The notebook begins with a summary of experiments that Greider had done so far. Greider set up an experiment with mated, spun, and lysed cells. Greider then mixed the cells to create a pellet of nucleate. With the nucleate Greider began testing different extracts for it and set up reactants, specifcally, G4T2 and NTP. Then ran the product through the gel and the bands were fuzzy. In two particular invitro experiments, Greider tested both hot and cold rNTP’s and fit them into tubes and then ran the tubes through a titration.

Greider found that the rNTP was not well incorporated into the base pair pattern of nucleotides in the DNA segement. She began testing different possibilities in order to figure out what the rNTP is doing in the process. In the end Greider ran the segment through a gel. Greider loaded a different gel this time using specific extracts, reactions, nucleotides. After the experiment, Greider tried to determine how much excess DNA she was adding. She concluded the notebook by reviewing the different preparations to extract DNA. Greider showcased the measurements of buffer solutions, reactants, cell mating processes, and suspension.

Preferred Citation

Laboratory Notebook 4: In Vitro 56-80/FR 20-40/RNA 10-21, 1985 - 1986. Carol Greider Collection, Cold Spring Harbor Laboratory Archives Digital Repository. 88-1525498. Update 2025-03-18.

Credit Line

Courtesy of Cold Spring Harbor Laboratory Archives.

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Category

Lab Notebooks

Clippings

Correspondence

Level

file

Collection

Carol Greider Collection

Resource ID

CWG

Component Unique Identifier

CWG_01_007

Reference ID

76b02b40cd9b55793968b8c46b3132dd

Finding Aid Link

Laboratory Notebook 4: In Vitro 56-80/FR 16-41/RNA 1-21