This is a notebook within a series on Greider's course notes. The notebook begins wth course notes, entitled Molecular Biology of Eukaryotic Microorganisms.The course instructor was Elizabeth Blackburn. The class had weekly discussion sections on key papers. The documents also contained a syllabus with necessary books and journal papers for the students. The notebook also contained diagrams of vegetative and mated cells fissioning. Greider noted that Tetrahymena cells mate simple cycle and have a total of seven mating types. The notebook also diagrammed out what mating looks like with Tetrahymena cells with a combined macronucleus and micronucleus. She also noted the macronuclear phenotypic assortment, especially the assortment alleles and conjugation of Tetrahymen cells.
Greider's work also contained a complete diagrams of eukaryotic micro-organisms. The micro-organisms included Paramecium, Tetrhymena, and Euplotes. Greider's course notes also discuss the genetics of Tetrahymena, specifcally haploid and diploid variaties.
An interesting note regarding Greider's Blackburn lecture entitled, Properties of Nuclei of Representative Ciliates. Greider's lecture notes discuss chromosome number and average DNA size of micornucleaus and macronucleaus for both Tetrahymena and Paramecium. As well as macronuclear development in ciliates and rearrangement of Tetrahymena genome. Greider believed that the telomere could be associated for sequence conservation. Also notes that telomeres seem to be conserved between different species among eukaryotes. Greider was also asking questions on how are telomeres made in her course notes. The Blackburn lecture also contained a literature review of variation of Trypanosomes as well as drawing graphs of variations of parasiticism in Trypanosoma. The notebook also contained notes on kDNA.
Greider also contained a course, The Cell Cycle: from Yeast to Humans. The course instructors were Bochan and Gerhart. The course contains normal cell cycle notes. The cell cylce of most animals cycle through Growth 1, Synthesis, Growth 2, and finally the Mitosis phase. The notebook also contains notes on cellular fusion and notes on Physarum, a slime mold, its cell cyle and a diagram of the cell cycle. Greider photocopied pictures of different stages of cell cycle. The pictures also had a detailed diagram of centrioles involved in Mitosis.
The notebook concluded a series of gel studies on cDNA. Greider found that there was a contaminated restriction enzyme that was degrading her DNA in certain columns when she sent it through the gel. The notebook also contained a letter from Blackburn to Greider. Greider and Blackburn were discussing 5 prime and 3 prime oligonucleotides for double stranded experiments. Greider thought they should focus on cloning experiments where they ligated and inserted vectors. Greider also tested the efficiency of G4T2 addition at various temperature. Blackburn responseded and did not think certain experiments were needed. Blackburn thought Greider should wait on getting oligos made untila basic reaction could be created.
Grieder responded saying that she gave group meeting where she took a video that she will send to Blackburn. Greider would initiate a Polymerase Chain Reaction experiment as soon as possible. In the end of the notebook there wasa a written note from Greider. Greider was sending Blackburn notes on how to clone RNA and thought the procedure was a little complicated. Greider does a Polymerase Chain Reaction on cDNA and Oligonucleotide. Greider also conducts gel studies which seem to be running better after obtaining restriction enzymes from a colleague. Greider ended the notebook with a series of transformation experiments on cells with labeled images.
Scope and Contents
This is a notebook within a series on Greider's course notes. The notebook begins wth course notes, entitled Molecular Biology of Eukaryotic Microorganisms.The course instructor was Elizabeth Blackburn. The class had weekly discussion sections on key papers. The documents also contained a syllabus with necessary books and journal papers for the students. The notebook also contained diagrams of vegetative and mated cells fissioning. Greider noted that Tetrahymena cells mate simple cycle and have a total of seven mating types. The notebook also diagrammed out what mating looks like with Tetrahymena cells with a combined macronucleus and micronucleus. She also noted the macronuclear phenotypic assortment, especially the assortment alleles and conjugation of Tetrahymen cells.
Greider's work also contained a complete diagrams of eukaryotic micro-organisms. The micro-organisms included Paramecium, Tetrhymena, and Euplotes. Greider's course notes also discuss the genetics of Tetrahymena, specifcally haploid and diploid variaties.
An interesting note regarding Greider's Blackburn lecture entitled, Properties of Nuclei of Representative Ciliates. Greider's lecture notes discuss chromosome number and average DNA size of micornucleaus and macronucleaus for both Tetrahymena and Paramecium. As well as macronuclear development in ciliates and rearrangement of Tetrahymena genome. Greider believed that the telomere could be associated for sequence conservation. Also notes that telomeres seem to be conserved between different species among eukaryotes. Greider was also asking questions on how are telomeres made in her course notes. The Blackburn lecture also contained a literature review of variation of Trypanosomes as well as drawing graphs of variations of parasiticism in Trypanosoma. The notebook also contained notes on kDNA.
Greider also contained a course, The Cell Cycle: from Yeast to Humans. The course instructors were Bochan and Gerhart. The course contains normal cell cycle notes. The cell cylce of most animals cycle through Growth 1, Synthesis, Growth 2, and finally the Mitosis phase. The notebook also contains notes on cellular fusion and notes on Physarum, a slime mold, its cell cyle and a diagram of the cell cycle. Greider photocopied pictures of different stages of cell cycle. The pictures also had a detailed diagram of centrioles involved in Mitosis.
The notebook concluded a series of gel studies on cDNA. Greider found that there was a contaminated restriction enzyme that was degrading her DNA in certain columns when she sent it through the gel. The notebook also contained a letter from Blackburn to Greider. Greider and Blackburn were discussing 5 prime and 3 prime oligonucleotides for double stranded experiments. Greider thought they should focus on cloning experiments where they ligated and inserted vectors. Greider also tested the efficiency of G4T2 addition at various temperature. Blackburn responseded and did not think certain experiments were needed. Blackburn thought Greider should wait on getting oligos made untila basic reaction could be created.
Grieder responded saying that she gave group meeting where she took a video that she will send to Blackburn. Greider would initiate a Polymerase Chain Reaction experiment as soon as possible. In the end of the notebook there wasa a written note from Greider. Greider was sending Blackburn notes on how to clone RNA and thought the procedure was a little complicated. Greider does a Polymerase Chain Reaction on cDNA and Oligonucleotide. Greider also conducts gel studies which seem to be running better after obtaining restriction enzymes from a colleague. Greider ended the notebook with a series of transformation experiments on cells with labeled images.
Preferred Citation
Notebook: cDNA/Cloning/Berkeley, 1986 - 1987. Carol Greider Collection, Cold Spring Harbor Laboratory Archives Digital Repository. 88-1525532. Update 2025-03-18.
Credit Line
Courtesy of Cold Spring Harbor Laboratory Archives.
Conditions Governing Access
Physical access by appointment only. Restrictions apply, see the CSHL Archival Access Policy or an archivist for details.
Conditions Governing Use
Consult our Permission & Fees Schedule page to determine the cost and restrictions of using materials in the archival collections.