Begins with a Tetrahymena ribosmoal DNA subsequence series and corresponding restriction enzyme Library Map
Also has a DNA Oligomer Request Form by Greider (18 oligomer) of nucleotides. Thymadine and Guanine. She ran more Southern blots and cut more Tet DNA for blots. Then Depurified blot gels and hybridized all 5 blots at 45 degrees Celcius. However she realized that she should have added a probe before hybradizing. Conducts several ligation tests using Hind enzyme and several yeast blot tests. Documents of note is a correspondence from Garnet at Berkley to Greider, describing his cell colony, treated it through denaturing, drying, and neutralizing the colony.
Scope and Contents
Begins with a Tetrahymena ribosmoal DNA subsequence series and corresponding restriction enzyme Library Map
Also has a DNA Oligomer Request Form by Greider (18 oligomer) of nucleotides. Thymadine and Guanine. She ran more Southern blots and cut more Tet DNA for blots. Then Depurified blot gels and hybridized all 5 blots at 45 degrees Celcius. However she realized that she should have added a probe before hybradizing. Conducts several ligation tests using Hind enzyme and several yeast blot tests. Documents of note is a correspondence from Garnet at Berkley to Greider, describing his cell colony, treated it through denaturing, drying, and neutralizing the colony.
Preferred Citation
Laboratory Notebook: "RNA Oligo" Blots, Cloning DCG1, 1987 - 1988. Carol Greider Collection, Cold Spring Harbor Laboratory Archives Digital Repository. 88-1525506. Update 2025-03-18.
Credit Line
Courtesy of Cold Spring Harbor Laboratory Archives.
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